Remineralisation science and daily prevention: Study adds evidence on biomimetic enamel care

A recent in vitro study compared three remineralising agents regarding their ability to support mineral recovery in artificially demineralised enamel lesions. Mineral recovery was assessed by calculating the calcium/phosphorus (Ca/P) ratio using energy-dispersive X-ray spectroscopy in conjunction with scanning electron microscopy (SEM), and surface morphology was evaluated using SEM. The study compared casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), zinc hydroxyapatite (Zn-HA) and fluoridated amorphous calcium phosphate (F-ACP), which is used in the Curasept Biosmalto professional mousse tested in the study.

The findings showed that all the tested formulations promoted a time-dependent remineralisation effect and that mineral recovery progressed over time. This confirmed that remineralisation is a gradual process requiring consistent application. However, differences between the formulations became evident during the evaluation period.

On the seventh day, the Zn-HA formulation demonstrated higher Ca/P values, suggesting an early mineral response. From the 14^th day, however, the F-ACP formulation demonstrated significantly greater mineral gain compared with the other agents. By the 21^st day, the F-ACP group showed Ca/P values approaching those of sound enamel. In comparison, CPP-ACP and Zn-HA demonstrated lower mineral recovery and reached a plateau earlier.

The SEM observations supported these findings. While CPP-ACP and Zn-HA showed partial surface recovery, the F-ACP group demonstrated a denser and more homogeneous enamel-like surface structure after 28 days, suggesting enhanced surface organisation following prolonged exposure.

From a clinical perspective, these findings reinforce two important principles for dental hygienists: the choice of remineralising technology matters, and remineralisation requires regular use over time. Remineralisation is not an immediate event; it is a biological process supported by consistent exposure and effective home care routines.

The study, titled “Time-effect comparative evaluation of three remineralizing agents on artificial enamel lesions: A SEM-EDX in vitro study”, was published online on 19 October 2025 in Journal of Clinical Medicine. More information about Curasept Biosmalto can be found at curasept.com. Curasept is a platinum sponsor of the 2026 International Symposium on Dental Hygiene.